The advantage of the CCK-8 test kit is that it does not require DMSO to dissolve, and is non-toxic, and operates.
Safe again
Convenient, it is very convenient and efficient for cell activity detection, cell proliferation and toxicity detection.
Details of the operation of the CCK-8 test kit in these areas, and how to choose the appropriate CCK-8 test kit?
CCK-8 Test Kit -
Cell activity assay
1. Inoculate a cell suspension (100 μl/well) in a 96-well plate. Place the plate in the incubator for a period of time (37 ° C, 5% CO)
2
).
2. Add 10 μl of CCK-8 solution to each well (be careful not to create bubbles in the wells that will affect the OD reading).
3. Incubate the plates in the incubator for 1-4 hours.
4. Determine the absorbance at 450 nm using a microplate reader.
Note: If the OD value is not measured temporarily, you can add it to each well.
10 μl 0.1 M HCL solution or 1% w/v SDS solution
And cover the culture plate in the dark to save at room temperature. The absorbance does not change within 24 hours.
CCK-8 Test Kit -
Cell proliferation-toxicity test
1. Dispense 100 μl of cell suspension (2000-5000 cells) in a 96-well plate. Place the plate in the incubator for 24 hours (37 ° C, 5% CO)
2
).
2. Add 10 μl of different concentrations of the test substance to the plate.
3. Incubate the plates in the incubator for a suitable period of time (eg, 6, 12, 24 or 48 hours).
4. Add 10 μl of CCK-8 solution to each well (do not create bubbles in the wells that will affect the OD reading).
5. Incubate the plates in the incubator for 1-4 hours.
6. Determine the absorbance at 450 nm using a microplate reader.
Note: If the OD value is not measured temporarily, you can add it to each well.
10 μl 0.1 M HCL solution or 1% w/v SDS solution
And cover the culture plate in the dark to save at room temperature. The absorbance does not change within 24 hours. If the substance to be tested is oxidizing or reducing, replace the medium with fresh medium (removal of the medium and wash the cells twice with medium, then add new medium) to remove the effects of the drug. Of course, in the case where the influence of the drug is relatively small, the medium can be directly subtracted from the blank absorption after the drug is added to the medium.
How to choose the right CCK-8 test kit?
The [CCK-8] cell proliferation/toxicity test kit currently available on the market is available in domestic or imported products. The domestically packaged CCK-8 kit has insufficient quality stability. The CCK-8 kits, which are native to domestic laboratories, have some good test results and are difficult to control in batch stability. As for the imported brands that supply CCK-8 kits, there are not many imported brands. Even Abcam, Thermo, Sigma and other brands can not provide CCK-8 kits, while other brands are far less expensive than Abbkine products.
As the first cell analysis flagship product of the Abbkine brand,
Cell Counting Kit (CCK-8)
Abbkine R&D engineers have invested a lot of money in continually exploring and optimizing the best formulas. Each effort is only to make scientific research workers more simple, efficient and stable access to valuable scientific research data.
"CCK-8 Test Kit: Comprehensive Comparison of Cell Proliferation and Toxicity Detection"
As the general agent of Abbkine China, Amy Jiejie will provide customers with the most cost-effective routine immunological reagents, such as various tag antibodies, internal reference antibodies, conventional primary antibodies and a full range of secondary antibodies, antibody detection substrates, protein purification products and Related kits, etc. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line.
400-6800-868
Contact Amytech Co., Ltd. for product information and complete experimental solutions.
LED PCB
The most comprehensive introduction of LED PCB
The relationship between LED and PCB
The printed circuit board is a genius innovation of circuit technology, laying less space and materials for more complex and large-scale circuits. The printed circuit board is such an important invention in circuit technology because it makes electrical and electronic components smaller, more flexible and more precise. For example, FPC(Flexible PCB), Multilayer PCB, BGA PCB , rigid-flex PCB and so on.
Soldering the LED chip to the PCB board, as long as the PCB circuit is connected in some way, it will illuminate, which forms the LED PCB. LED PCBs typically also have a heat sink and ceramic pedestal to hold the circuit in place and allow the smooth operation of the circuit. A heat sink is required because LEDs tend to generate a lot of heat, which can damage the PCB and the equipment that connects the circuits.
Because LED will produce a lot of heat, led PCB generally uses metal substrate as its production material. Metals with the ability to dissipate heat quickly have proved to be a better choice. Generally, whenever led PCB is designed, Aluminum PCB is preferred. Aluminum PCB usually also contains a thin layer of dielectric, which can dissipate heat faster.
In addition to general lighting, led PCB can also provide visible light, infrared and ultraviolet light. Compared with other light sources, LED PCB can provide higher energy.
We are a professional LED PCB manufacturer and assembler in China, In this article, we would like to introduce in detail what is LED PCB, the materials used in manufacturing and LED circuit boards manufacturing, the application of LED printed circuit boards and so on.
In China, there are many PCB manufacturers and suppliers. Jinghongyi PCB is one of the LED PCB board manufacturers. We only provide LED PCB fabrication services. LED PCB design is not our specialty and business scope.
LED PCB board Introduction
LED PCB has many other names, such as LED PCB board, LED circuit, LED circuit board, led printed circuit boards, PCB board for LED.
In a sense, LED PCB and aluminum PCB belong to the same kind of PCB products, because their uses and materials are mostly the same.
With the popularization and widespread use of industrial lighting and civil lighting, whether outdoor or indoor, family or commercial buildings and factories, lighting provides the guarantee of its normal operation.
Of course, nowadays, with the development of human society, environmental pollution has become a difficult problem in the world. In order to reduce the consumption of coal, such as coal used for power generation in thermal power plants, coal combustion pollutes the atmosphere very seriously. In order to save energy and protect the environment, LED lights and previous lighting methods have great advantages, except for energy. Besides providing brighter lights, it also contributes to environmental protection.
LED PCB Meaning
LED circuit board is a kind of printed circuit board.
LED PCB Board Manufactureing
Both LED aluminium PCB and FR-4 fiberglass circuit board belong to PCB. To say differently, only compare LED aluminium substrate with FR-4 fiberglass circuit board.
LED aluminium substrate is printed on the surface of aluminium material with better thermal conductivity, and then weld electronic components on it.
In electronics, an LED circuit or LED driver is an electrical circuit used to power a light-emitting diode (LED). The circuit must provide sufficient current to light the LED at the required brightness, but must limit the current to prevent damaging the LED.
The voltage drop across an LED is approximately constant over a wide range of operating current; therefore, a small increase in applied voltage greatly increases the current.
Very simple circuits are used for low-power indicator LEDs. More complex, current source circuits are required when driving high-power LEDs for illumination to achieve correct current regulation.
An LED PCB board tends to generate a high volume of heat, making it difficult to cool via traditional means.
LED PCB Board Material - LED PCB raw material, LED bulb PCB raw material, LED strip PCB material
Metal core PCBs are often chosen for LED applications because they have enhanced heat dissipation capabilities. In particular, aluminium is usually used to make circuit boards for LED lamps. Aluminum substrates usually consist of thin layers of thermal conductive dielectrics that can be transported and have much more efficient heat dissipation than conventional rigid PCBs.
Compared with other electronic components, LED generates more heat, which requires PCB to heat very well. For this special feature, Metal Core PCB is often used in LED circuit boards, especially aluminum core PCB.
The following is the heat dissipation capacity of PCB plates of different materials, from weak to strong
1. FR4, common PCB board material;
2. Alumina substrate;
3. There is no doubt that the thermal conductivity of ceramic PCB is the best among many materials;
4. The thermal conductivity of alumina ceramic circuit board can be about ten times that of the aluminum substrate;
5. The thermal conductivity of Slyton aluminum nitride ceramic circuit board is ten times higher than that of alumina;
In this kind of ultra-high thermal conductivity, LED light attenuation will be greatly weakened, and its service life will be greatly increased.
LED Lighting PCB Assembly
Seeking LED PCB and Assembly? LED PCB manufacturing and assembly; Led board made based on your PCB files. Turn-key services; 100% inspection.
Applications of LED PCB
PCB LED lights can be incorporated into numerous lighting applications due to their combination of excellent energy efficiency, low cost and maximum design flexibility.
- Automotive headlights
- Airport runway landing lights
- Lighting used in military field
- Street lighting
- Highway tunnel lighting
- Photovoltaic (solar) lighting
- Flashlights and lanterns
- Traffic and signal lighting
- Lighting in hospital operating rooms
- High growth plant lighting
- Highway tunnel lighting
- Solar power products
If you have any questions about LED PCB Board, Please feel free to send us email. If you have final Gerber for production, welcome your inquiry.
Additional Information
Aluminum PCB for LED Light/Lamp/Tube
Aluminum PCB for LED
Aluminium Base LED PCB
LED Metal Core PCB
Metal Core PCB for LED
PCB industry cut into LED PCB heat sink aluminum substrate
Double Sided Aluminum PCB
LED PCB,LED Circuit Board,LED PCB Board,LED Printed Circuit Board
JingHongYi PCB (HK) Co., Limited , https://www.pcbjhy.com