Fish 5 nucleotidease detection kit - Huaqiang Electronic Network

Test - lowercase jpg

Kaixin micro test

Test probe P100-M3

1.450±10nm filter microplate reader (recommended preheating before use)

2. High-precision sampler and gun head: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL.

3.Eppendorf pipette.

4. Distilled or deionized water.

5. absorbent cotton absorbent paper.

6.37 ° C incubator.

7.100ml and 1000ml graduated cylinders.

8. Prepare several standard dilution tubes.

Storage and expiration date of the kit:

Unopened kit: All reagents are stored as indicated on the reagent bottle label. Please note that after receiving the kit, please store the TMB washing solution and the stop solution at 4 °C as soon as possible, and store the other reagents at -20 °C.

After use of the kit: the remaining reagents should still be stored according to the temperature indicated on the label of the reagent bottle. After the opening, the enzyme labeling plate should be sealed and stored at -20 °C to avoid moisture.

Experimental principle:

The target antibody is coated in a 96-well microplate to prepare a solid phase carrier, and a standard or a sample is separately added to the microwell, wherein the antibody linked to the solid phase carrier is bound, and then the microbial target antibody is added. After washing the unbound biotin antibody, HRP label and avidin were added, washed thoroughly again, and TMB substrate was added for color development. TMB is converted to blue under the catalysis of peroxidase and converted to the final yellow color by the action of an acid. The depth of the color is positively correlated with the target in the sample. The absorbance (OD value) was measured at a wavelength of 450 nm using a microplate reader to calculate the sample concentration.

Precision:

The precision is expressed by the coefficient of variation CV of the measured value of the sample. CV (%) = SD / mean × 100

Batch internal difference: Take the same batch of kits for quantitative detection of low, medium and high value fixed samples. Each sample is continuously measured 20 times, and the average value and SD value of different concentration samples are calculated separately.

Batch-to-batch difference: Select three different batches of kits to quantitatively determine low, medium and high value fixed samples. Each sample is repeatedly measured 8 times using the same kit, and the average value and SD value of different concentration samples are calculated separately. .

Batch difference: CV<10% Inter-batch difference: CV<13%

stability:

It has been determined that the kit is stored at the recommended temperature during the period of validity, and its activity reduction rate is less than 5%.

In order to reduce the influence of external factors on the detection values ​​before and after the damage of the kit, the environmental conditions of the laboratory should be kept as consistent as possible, especially the temperature, humidity and incubation conditions in the laboratory. Secondly, the same experimenter can operate to reduce human error.

Friendly reminder:

After using the kit, please dispose of the consumables properly to avoid environmental pollution!