Related antibody reaction rule in Elisa kit experiment - Database & Sql Blog Articles

Reaction law of related antibodies in elisa kit experiment

The basis of ELISA is the immobilization of antigen or antibody and the enzymatic labeling of antigen or antibody. The antigen or antibody bound to the surface of the solid support still retains its immunological activity, and the enzyme-labeled antigen or antibody retains its immunological activity, and Retaining the activity of the enzyme. At the time of measurement, the test specimen (the antibody or antigen therein is reacted) reacts with the antigen or antibody on the surface of the solid phase carrier. The antigen-antibody complex formed on the solid phase carrier is mixed with the liquid by washing. The other substances are separated. The enzyme-labeled antigen or antibody is further added to the solid phase carrier by the reaction. At this time, the amount of the enzyme on the solid phase is proportional to the amount of the test substance in the sample. After the substrate, the substrate is catalyzed by the enzyme to become a colored product. The amount of the product is directly related to the amount of the substance to be tested in the sample, so that it can be qualitatively or quantitatively analyzed according to the depth of the color. Due to the high catalytic efficiency of the enzyme, indirectly Amplified the results of the immune response, making the measurement method achieve high sensitivity. Shanghai Jinma Bioanalysis

The reaction law of related antibodies in the Elisa kit experiment, welcome to read:

(1) The initial reaction produces antibodies: When the antigen enters the body for the first time, it takes a certain incubation period to produce antibodies, and the amount of antibodies produced is not much, and the time spent in the body is also short. (2) Re-reaction to produce antibodies: When the same antigen enters the body for the second time, at the beginning, since a part of the original antibody binds to the re-entered antigen, the amount of the original antibody is slightly lowered. Subsequently, the antibody titer rapidly increases in a large amount, which is several times to several tens of times larger than that produced by the initial reaction, and remains in the body for a longer period of time. (3) Recalling the reaction to produce antibodies: The antibody produced by the antigen to stimulate the body gradually disappears after a certain period of time. At this time, if the antigen is contacted again, the disappeared antibody can be rapidly raised. If the antigen that re-stimulates the body is the same as the first time, it is called a specific recall reaction; if it is different from the initial reaction, it is called a non-specific recall reaction. The rise of antibodies caused by non-specific recall reactions is temporary, and the company will provide high-quality, affordable ELISA kits in a short period of time. If you need friends, please contact us by phone, QQ or mobile phone! ELISA kit specifications: 96T/48T