Nanjing Jinyibai Company explained to everyone: the best way to preserve serum should be stored in -5C to -2OC. However, if stored at 4C, do not exceed one month. If you are unable to use one bottle at a time, it is recommended to aliquot the serum into a suitable sterile container and return it to the freezer.
Serum refers to a light yellow transparent liquid separated from fibrin in plasma or a plasma in which fibrin has been removed after blood coagulation. Its main role is to provide basic nutrients, provide hormones and various growth factors, provide binding proteins, provide pro-contact and stretching factors to protect cells from mechanical damage, and protect cells in culture.
The following points should be noted for the preservation of serum:
(1) Serum that needs to be stored for a long time must be stored in a low temperature refrigerator at -20 ° C - 70 ° C. The storage time in the refrigerator at 4 ° C must not exceed 1 month. The volume will increase by about 10% due to serum icing, therefore, the serum is Before freezing into a low-temperature refrigerator, a certain volume of space must be reserved, otherwise it is prone to pollution or glass bottle cracking.
(2) Heat inactivation refers to the freezing of serum that has been completely thawed at 56 ° C for 30 minutes. It should be shaken regularly during heating. The purpose of this heat treatment is to inactivate the complement in the serum. Unless necessary, generally not This heat treatment is recommended because heat treatment will cause a significant increase in serum sediments, and will also affect the quality of serum. Complement is involved in the reaction: cytotoxicity, smooth muscle cell contraction, mastamine and platelet release of histamine, enhance phagocytosis, promote lymphatics Cells and macrophages undergo chemotactic and activation.
(3) The thawing of bottled serum should be carried out by gradual thawing method: -20 ° C to -70 ° C. The serum in the low temperature refrigerator is dissolved in a refrigerator at 4 ° C for 1 day. Then it is transferred to room temperature, and then completely dissolved before being dispensed. During the dissolution process It should be gently shaken evenly (be careful not to cause bubbles), so that the temperature and composition are uniform, and the precipitation is reduced. Do not directly thaw the serum from -20 ° C into 37 ° C, so that the temperature changes too much, which is easy to cause protein aggregation. Precipitation occurred.
(4) The serum provided by the general manufacturer is sterile and does not need to be filtered and sterilized. If serum is found to be suspended, the serum can be added to the culture solution for filtration, and the serum should not be directly filtered.
(5) Precipitate floc in serum: mainly caused by denaturation of serum lipoprotein and fibrin in serum after thawing. These flocs do not affect the quality of serum itself. It can be removed by centrifugation at 3000 rpm for 5 minutes. Can be used without treatment. Microscopic "small black spots": After heat-treated serum, the formation of precipitates will increase significantly. Some precipitates are observed under the microscope like "small black spots", often mistaken for serum contamination. Under normal circumstances This small black spot will not affect cell growth, but if serum quality is suspected, stop using it immediately and replace another batch of serum.
(6) Do not leave the serum at 37 °C for too long, otherwise the serum will become turbid, and the active ingredients in the serum will break and affect the serum quality.
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