What are the differences between reagent blanks and sample blanks?

A reagent blank is a small positive error in the test results due to the test reagent itself. Hali is constantly trying to make the blank of its test reagents negative, especially because this negative blank is so small that it does not affect the accuracy of the assay. The blank value of the test reagent is very important for a test. When performing low concentration control, the blank value of the test reagent should be deducted from the test result. For example, if the reagent blank value of 0.02 mg/L is subtracted from the low concentration test result of 0.06 mg/L, the final measurement result will be changed by at least 30%. By subtracting the reagent blank value of 0.02 mg/L from the high concentration test of 1.23 mg/L, only 2% of the change was observed. When measuring the blank value of the reagent, it is necessary to use high-quality deionized water instead of the sample to be tested, participate in the test reagent, and perform a routine test according to the operation steps. Then, the blank value of the test reagent is subtracted from the measurement result of the sample. The blank value of the different batches of test reagents will change, so when using different batches of test reagents, it is necessary to determine the blank value of the batch from the beginning. Sample blank refers to the concentration of a sample used as the zero reference of the instrument in a test procedure. The sample blank can offset the positive error caused by the chroma or turbidity of the sample itself before participating in the test reagent. After zeroing the test instrument using the sample blank, the chromaticity that occurs as long as the sample reacts with the test reagent is determined. Because the background color and turbidity of the sample tend to be different, sample blanks are often used to zero the instrument.

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