Specimen preparation must be standardized. The volume of each specimen should be prepared (stored) according to the amount of 2-3 or more duplicates. Those who are unable to experiment in the short term should pay attention to cryopreservation;
Prepare all additional items required for the experiment, such as test tubes, tips, pipettes, centrifuges, etc.; determine the amount of reagents required based on the number of test specimens; finally, equilibrate the reagents used to room temperature according to the instructions to prepare the required reagents.Be sure to pay attention to the preparation before the experiment, which is crucial to the success of the experiment, the details determine success or failure.
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